5CFF

Crystal structure of Miranda/Staufen dsRBD5 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2013-10-28
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	C 1 2 1
Unit cell lengths	200.023, 51.332, 100.274
Unit cell angles	90.00, 90.56, 90.00

Refinement procedure

Resolution	27.664 - 2.500
R-factor	0.2443
Rwork	0.242
R-free	0.28100
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.183
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELX
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.540
High resolution limit [Å]	2.500	6.780	2.500
Rmerge	0.078	0.045	0.664
Total number of observations	189671
Number of reflections	34191
<I/σ(I)>	13.9
Completeness [%]	94.9	88.1	99.8
Redundancy	5.5	4.8	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.8	289	sodium acetate,1,6-hexanediol,calcium chloride etc