5CEZ

Crystal Structure of the BG505 SOSIP gp140 HIV-1 Env trimer in Complex with an early putative precursor of the PGT121 family at 3.0 Angstrom

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-11
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.033
Spacegroup name	P 63
Unit cell lengths	127.978, 127.978, 316.124
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.493 - 3.031
R-factor	0.235
Rwork	0.233
R-free	0.26560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4r26 4tvp
RMSD bond length	0.006
RMSD bond angle	1.032
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.000	3.000
Number of reflections	56534
<I/σ(I)>	13.6
Completeness [%]	100.0	100
Redundancy	6.3	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277.15	0.2 M CaCl2, 28% (v/v) PEG 400 and 0.1 M Hepes pH 7.5