5CEB
Bd3459 Predatory Endopeptidase from Bdellovibrio bacteriovorus, K38M form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-11-30 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.91739 |
| Spacegroup name | P 1 |
| Unit cell lengths | 55.930, 65.040, 73.816 |
| Unit cell angles | 63.89, 83.27, 83.20 |
Refinement procedure
| Resolution | 66.110 - 1.930 |
| R-factor | 0.1976 |
| Rwork | 0.196 |
| R-free | 0.23220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3v39 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.629 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 100.000 |
| High resolution limit [Å] | 1.930 |
| Number of reflections | 56655 |
| <I/σ(I)> | 16.2 |
| Completeness [%] | 85.7 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.1M potassium thiocyanate, 30% w/v PEG 2000 MME |
