5CE8
Crystal structure of branched-chain aminotransferase from thermophilic archaea Thermoproteus uzoniensis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-15 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 32 1 2 |
| Unit cell lengths | 93.540, 93.540, 212.510 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 75.690 - 2.000 |
| R-factor | 0.1222 |
| Rwork | 0.120 |
| R-free | 0.16810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2eiy |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.396 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 75.690 | 2.100 | |
| High resolution limit [Å] | 2.000 | 80.000 | 2.000 |
| Rmerge | 0.119 | 0.679 | |
| Rmeas | 0.123 | 0.697 | |
| Total number of observations | 1413383 | ||
| Number of reflections | 72105 | 9728 | |
| <I/σ(I)> | 19.28 | 4.77 | |
| Completeness [%] | 99.9 | 99.9 | |
| Redundancy | 19.6 | 19.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 0.1 M Sodium acetate trihydrate pH 4.6, 5% w/v PEG 4,000 |
