5CE3
The Yersinia YopO - actin complex with MgADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2013-09-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97626 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 108.730, 121.750, 118.580 |
| Unit cell angles | 90.00, 104.88, 90.00 |
Refinement procedure
| Resolution | 114.600 - 2.930 |
| R-factor | 0.19807 |
| Rwork | 0.196 |
| R-free | 0.24024 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ci6 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.440 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 114.600 | 3.010 |
| High resolution limit [Å] | 2.930 | 2.930 |
| Rmerge | 0.125 | 0.778 |
| Number of reflections | 64212 | |
| <I/σ(I)> | 11.9 | 2.7 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 6.8 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 279.15 | 0.1M HEPES, pH 7.5, 30% PEG 400, 0.2M NaCl |
