5CDK

Apical domain of chloroplast chaperonin 60b1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	80
Detector technology	CCD
Collection date	2014-12-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 21 21 2
Unit cell lengths	63.866, 90.757, 31.551
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.551 - 1.500
R-factor	0.1407
Rwork	0.139
R-free	0.16590
RMSD bond length	0.018
RMSD bond angle	1.622
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	31.560
High resolution limit [Å]	1.490
Number of reflections	30082
<I/σ(I)>	37.8
Completeness [%]	99.9
Redundancy	14.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277.15	0.05 M Calcium chloride dehydrate, 0.1 M BIS-TRIS pH 6.5, 30% v/v Polyethylene glycol monomethyl ether 550