5CDJ
apical domain of chloroplast chaperonin 60a
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2015-06-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 |
| Unit cell lengths | 33.912, 53.024, 59.311 |
| Unit cell angles | 110.02, 106.32, 91.42 |
Refinement procedure
| Resolution | 32.330 - 1.750 |
| R-factor | 0.1869 |
| Rwork | 0.186 |
| R-free | 0.21060 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.781 |
| Data reduction software | CNS |
| Data scaling software | CNS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 32.330 |
| High resolution limit [Å] | 1.750 |
| Number of reflections | 35856 |
| <I/σ(I)> | 28 |
| Completeness [%] | 96.3 |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291.15 | 0.1 M Tris pH 8.5, 2.0 M ammonium sulfate |
