5CDI
Chloroplast chaperonin 60b1 of Chlamydomonas
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2013-07-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 143.338, 174.390, 213.678 |
| Unit cell angles | 90.00, 94.69, 90.00 |
Refinement procedure
| Resolution | 48.657 - 3.807 |
| R-factor | 0.276 |
| Rwork | 0.275 |
| R-free | 0.32540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xck |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.597 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.660 | 3.870 |
| High resolution limit [Å] | 3.807 | 3.800 |
| Rmerge | 0.970 | |
| Number of reflections | 101331 | |
| <I/σ(I)> | 18.27 | 18.27 |
| Completeness [%] | 96.7 | 93.6 |
| Redundancy | 4 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | 10% w/v Polyethylene glycol monomethyl ether 5000, 5% v/v Tacsimate pH 7.0, 0.1 M HEPES pH 7.0 |
