5CD5
Crystal structure of an immature VRC01-class antibody DRVIA7 from a Chinese donor bound to clade A/E HIV-1 gp120 core
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-16 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 72.325, 72.325, 338.750 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.968 - 3.396 |
| R-factor | 0.2688 |
| Rwork | 0.253 |
| R-free | 0.30600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.330 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.968 | 3.670 |
| High resolution limit [Å] | 3.390 | 3.390 |
| Rmerge | 0.310 | 2.920 |
| Number of reflections | 13362 | |
| <I/σ(I)> | 10 | 1.2 |
| Completeness [%] | 99.7 | 99.3 |
| Redundancy | 15.4 | 15.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.2 M potassium iodide and 20% (w/v) PEG 3350 |
