5CD2
The crystal structure of endo-1,4-D-glucanase from Vibrio fischeri ES114
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.563, 67.489, 101.192 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.500 - 1.550 |
| R-factor | 0.1374 |
| Rwork | 0.135 |
| R-free | 0.17600 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.025 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.500 | 1.580 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.104 | 0.602 |
| Number of reflections | 56160 | |
| <I/σ(I)> | 20.362 | 1.67 |
| Completeness [%] | 99.8 | 96.8 |
| Redundancy | 6.2 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 289 | 0.2M MgCl2, 0.1M Tris:HCl, 20%(w/v) PEG8000 |
