5CCX
Structure of the product complex of tRNA m1A58 methyltransferase with tRNA3Lys as substrate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 103 |
Detector technology | CCD |
Collection date | 2014-09-14 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.11587 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 136.770, 136.770, 177.160 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 54.130 - 2.100 |
R-factor | 0.208 |
Rwork | 0.207 |
R-free | 0.23700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ccb |
RMSD bond length | 0.004 |
RMSD bond angle | 0.699 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 108.000 | 2.150 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.103 | 2.590 |
Number of reflections | 98180 | |
<I/σ(I)> | 16.7 | 0.9 |
Completeness [%] | 99.9 | 100 |
Redundancy | 8.1 | 8.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 1 microliter protein solution (4.8 mg/ml protein, 66.4 micromolar tRNA3Lys, 2mM SAM, 1mM MgCl2 and 50mM Hepes pH 7.5) plus 1 microliter reservoir solution (0.1 M NaAcetate, pH 4.8-5.0, 2% w/v PEG 4000 and 15% v/v MPD) over 1000 microliters reservoir solution |