5CC2

STRUCTURE OF THE LIGAND-BINDING DOMAIN OF THE IONOTROPIC GLUTAMATE RECEPTOR-LIKE GLUD2 IN COMPLEX WITH 7-CKA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I911-2
Synchrotron site	MAX II
Beamline	I911-2
Temperature [K]	100
Detector technology	CCD
Collection date	2006-11-22
Detector	MAR CCD 165 mm
Wavelength(s)	1.04080
Spacegroup name	P 43 21 2
Unit cell lengths	70.210, 70.210, 134.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.577 - 2.501
R-factor	0.1951
Rwork	0.193
R-free	0.23930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2v3t molA
RMSD bond length	0.003
RMSD bond angle	0.656
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.9)
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.580	30.580	2.640
High resolution limit [Å]	2.501	7.910	2.500
Rmerge		0.033	0.519
Rmeas	0.080
Rpim	0.035	0.018	0.251
Total number of observations	62324	1599	9045
Number of reflections	12222
<I/σ(I)>	13.4	26.4	2.8
Completeness [%]	99.6	87.6	100
Redundancy	5.1	4	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	280	20 mM Hepes, 10 mm NaCl, 1 mM EDTA