5CAV
EGFR kinase domain with compound 41a
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 110 |
Detector technology | PIXEL |
Collection date | 2013-12-18 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97950 |
Spacegroup name | I 2 3 |
Unit cell lengths | 146.310, 146.310, 146.310 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 23.480 - 2.730 |
R-factor | 0.1984 |
Rwork | 0.197 |
R-free | 0.23130 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.050 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | BUSTER (2.11.4) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 23.500 |
High resolution limit [Å] | 2.700 |
Number of reflections | 13899 |
<I/σ(I)> | 19.4 |
Completeness [%] | 99.2 |
Redundancy | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 292 | sodium/potassium tartrate |