5CAE
Succinate bound to pig GTP-specific succinyl-CoA synthetase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-09-20 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.97947 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 86.670, 81.020, 50.780 |
| Unit cell angles | 90.00, 104.90, 90.00 |
Refinement procedure
| Resolution | 49.073 - 2.200 |
| R-factor | 0.1695 |
| Rwork | 0.165 |
| R-free | 0.21360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xx0 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.542 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA (3.3.21) |
| Phasing software | PHENIX (1.9_1692) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.073 | 49.073 | 2.320 |
| High resolution limit [Å] | 2.200 | 6.960 | 2.200 |
| Rmerge | 0.028 | 0.466 | |
| Rmeas | 0.113 | ||
| Rpim | 0.058 | 0.017 | 0.284 |
| Total number of observations | 122021 | 3961 | 16605 |
| Number of reflections | 33544 | ||
| <I/σ(I)> | 9.3 | 20.7 | 2.6 |
| Completeness [%] | 97.0 | 97.8 | 94.6 |
| Redundancy | 3.6 | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 294 | PEG 3350, ammonium succinate, magnesium chloride, CoA, HEPES, pH 7.0 |
