5C97
Insulin regulated aminopeptidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-08-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.000, 260.151, 73.360 |
| Unit cell angles | 90.00, 111.61, 90.00 |
Refinement procedure
| Resolution | 68.204 - 3.370 |
| R-factor | 0.2198 |
| Rwork | 0.216 |
| R-free | 0.28530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4p8q |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.191 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.270 | 3.460 |
| High resolution limit [Å] | 3.370 | 3.370 |
| Rmerge | 0.058 | 0.720 |
| Number of reflections | 33870 | |
| <I/σ(I)> | 16.5 | 2.7 |
| Completeness [%] | 99.8 | 99.6 |
| Redundancy | 6.8 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 10% w/v PEG 4000, 20% v/v glycerol, 0.1M bicine/Trizma base, 0.2 M sodium L-glutamate, 0.2M DL-alanine, 0.2M glycine, 0.2M DL-lysine HCl, 0.2M DL-serine |
