5C90
Staphylococcus aureus ClpP mutant - Y63A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-03-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9735 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 94.359, 126.237, 145.647 |
| Unit cell angles | 90.00, 93.74, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.750 |
| R-factor | 0.18312 |
| Rwork | 0.182 |
| R-free | 0.20164 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3sta |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.466 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 340047 | |
| <I/σ(I)> | 16.6 | 3.8 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 3.4 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 30% MPD, 100mM sodium acetate (pH 5.5), 20mM calcium chloride |
