5C88

Crystal structure of Ard1 N-terminal acetyltransferase from Sulfolobus solfataricus in monoclinic form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-01-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	34.154, 84.246, 49.165
Unit cell angles	90.00, 89.88, 90.00

Refinement procedure

Resolution	26.637 - 2.490
R-factor	0.1714
Rwork	0.161
R-free	0.26520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4r3k
RMSD bond length	0.008
RMSD bond angle	1.159
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.580
High resolution limit [Å]	2.490	5.360	2.490
Rmerge	0.087	0.044	0.493
Total number of observations	31094
Number of reflections	9431
<I/σ(I)>	10.3
Completeness [%]	95.5	89.5	98.8
Redundancy	3.3	2.9	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.2	283	0.6 M ammonium sulfate, 0.1 M sodium acetate trihydrate, pH 4.2, 0.1 M lithium sulfate