5C88
Crystal structure of Ard1 N-terminal acetyltransferase from Sulfolobus solfataricus in monoclinic form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.154, 84.246, 49.165 |
| Unit cell angles | 90.00, 89.88, 90.00 |
Refinement procedure
| Resolution | 26.637 - 2.490 |
| R-factor | 0.1714 |
| Rwork | 0.161 |
| R-free | 0.26520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4r3k |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.159 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.580 |
| High resolution limit [Å] | 2.490 | 5.360 | 2.490 |
| Rmerge | 0.087 | 0.044 | 0.493 |
| Total number of observations | 31094 | ||
| Number of reflections | 9431 | ||
| <I/σ(I)> | 10.3 | ||
| Completeness [%] | 95.5 | 89.5 | 98.8 |
| Redundancy | 3.3 | 2.9 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 283 | 0.6 M ammonium sulfate, 0.1 M sodium acetate trihydrate, pH 4.2, 0.1 M lithium sulfate |
