5C80
X-ray structure uridine phosphorylase from Vibrio cholerae in complex with uridine at 2.24 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-16 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97989 |
| Spacegroup name | P 1 |
| Unit cell lengths | 61.255, 73.110, 83.137 |
| Unit cell angles | 71.81, 77.19, 85.69 |
Refinement procedure
| Resolution | 45.470 - 2.243 |
| R-factor | 0.1716 |
| Rwork | 0.168 |
| R-free | 0.23700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4lwz |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.532 |
| Data scaling software | SCALA (3.3.22) |
| Phasing software | PHASER (2.5.7) |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 77.231 | 45.427 | 2.360 |
| High resolution limit [Å] | 2.243 | 7.090 | 2.243 |
| Rmerge | 0.055 | 0.026 | 0.333 |
| Rmeas | 0.077 | ||
| Rpim | 0.055 | 0.026 | 0.333 |
| Total number of observations | 101646 | 3195 | 13271 |
| Number of reflections | 58293 | ||
| <I/σ(I)> | 9.6 | 26.4 | 2.3 |
| Completeness [%] | 91.1 | 92.3 | 83.2 |
| Redundancy | 1.7 | 1.7 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | PEG4000, 0.1M TRIS-HCl, 0.2M MgCl2x6H2O |
