5C7X
Crystal structure of MOR04357, a neutralizing anti-human GM-CSF antibody Fab fragment in complex with human GM-CSF
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-01-11 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 71.602, 97.493, 192.512 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 96.260 - 2.950 |
| R-factor | 0.23449 |
| Rwork | 0.234 |
| R-free | 0.27613 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gmf |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.166 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 96.260 | 3.180 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.180 | 0.437 |
| Number of reflections | 29123 | |
| <I/σ(I)> | 6.8 | 1.8 |
| Completeness [%] | 94.5 | 95.5 |
| Redundancy | 4.7 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 293 | 100 mM sodium acetate, pH 4.0-4.3, 39-42 % (w/v) PEG 400, 40 mg/mL protein |
