5C7W
5'-monophosphate Z:P Guanine Riboswitch bound to hypoxanthine.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-04-07 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 52.180, 52.180, 274.500 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.923 - 3.220 |
R-factor | 0.2308 |
Rwork | 0.228 |
R-free | 0.27970 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4fe5 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.802 |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.590 | 3.340 |
High resolution limit [Å] | 3.220 | 3.220 |
Rmerge | 0.083 | 0.537 |
Number of reflections | 4107 | |
<I/σ(I)> | 24.9 | 4.1 |
Completeness [%] | 99.8 | 100 |
Redundancy | 8.54 | 8.95 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 296 | 17% PEG 1000, 240 mM NH4OAc, 10 mM K-HEPES, 10 mM Co(NH3)6Cl3 |