5C7U
5'-monophosphate wt Guanine Riboswitch bound to hypoxanthine.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-03-22 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 52.600, 52.600, 278.120 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.553 - 3.050 |
| R-factor | 0.2034 |
| Rwork | 0.201 |
| R-free | 0.24110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fe5 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.663 |
| Data reduction software | HKL-2000 |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (dev_1405) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.360 | 3.160 |
| High resolution limit [Å] | 3.050 | 3.050 |
| Rmerge | 0.065 | 0.500 |
| Number of reflections | 4923 | |
| <I/σ(I)> | 33 | 5.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14 | 14.82 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 296 | 17% PEG 1000; 240 mM NH4OAc; 10 mM K-HEPES; 10 mM Co(NH3)6Cl3 |
