5C7L

Structure of human testis-specific glyceraldehyde-3-phosphate dehydrogenase apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2014-04-10
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0746
Spacegroup name	P 31 2 1
Unit cell lengths	86.888, 86.888, 159.606
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.156 - 1.862
R-factor	0.188
Rwork	0.186
R-free	0.22700
RMSD bond length	0.013
RMSD bond angle	1.399
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall
Low resolution limit [Å]	38.200
High resolution limit [Å]	1.860
Number of reflections	59104
<I/σ(I)>	17.5
Completeness [%]	99.8
Redundancy	8.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	273	mix 1:1 volume of protein at 10 mg/mL with 0.2 M sodium sulfate, 0.1 M Bis-Tris propane pH 6.5, 20% PEG 3350