5C7I

Mouse sperm Glyceraldehyde-3-phosphate dehydrogenase: apo enzyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2007-07-16
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.5418
Spacegroup name	P 31 2 1
Unit cell lengths	86.717, 86.717, 158.358
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.598 - 2.010
R-factor	0.1797
Rwork	0.178
R-free	0.20690
RMSD bond length	0.005
RMSD bond angle	0.922
Data reduction software	DENZO
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall
Low resolution limit [Å]	30.600
High resolution limit [Å]	2.010
Number of reflections	44704
<I/σ(I)>	23.7
Completeness [%]	95.9
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	293	1:1 mixture of protein at 3 mg/mL with 20% PEG3350, 0.1 M Bis-Tris-propane pH 6.5, 0.2 M potassium thiocyanate.