5C7G
Crystal Structure of the b1 Domain of Human Neuropilin-1 in complex with a bicine molecule
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97967 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 62.280, 62.280, 85.960 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.278 - 1.450 |
| R-factor | 0.1435 |
| Rwork | 0.141 |
| R-free | 0.17250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kex |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.267 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.200 | 1.490 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.049 | 0.527 |
| Number of reflections | 30515 | |
| <I/σ(I)> | 24.9 | 3.9 |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 12 | 10 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 25% PEG550 mme 5%, PEG 20 000, 60mM MgCl2, Na-Bicine 100 mM, pH 8.5 |
