5C6R
Crystal structure of PH domain of ASAP1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-03-01 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 43 |
Unit cell lengths | 48.120, 48.120, 111.333 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.120 - 1.800 |
R-factor | 0.21068 |
Rwork | 0.208 |
R-free | 0.25986 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2da0 |
RMSD bond length | 0.018 |
RMSD bond angle | 1.999 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.800 |
Number of reflections | 22383 |
<I/σ(I)> | 15.6 |
Completeness [%] | 95.4 |
Redundancy | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 296 | 100 mM Na2HPO4, 100 mM Tris, pH 8.0 and 48% PEG400 (v/v) |