5C6G

Structural Insights into the Scc2-Scc4 Cohesin Loader

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-10-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.92
Spacegroup name	P 1 21 1
Unit cell lengths	112.240, 88.810, 116.000
Unit cell angles	90.00, 118.49, 90.00

Refinement procedure

Resolution	98.648 - 2.600
R-factor	0.2297
Rwork	0.227
R-free	0.27430
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.231
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	SHARP
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	98.660	2.620
High resolution limit [Å]	2.550	2.550
Rmerge	0.040	0.591
Number of reflections	63440
<I/σ(I)>	17.96
Completeness [%]	96.8
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	PEG 6000, cacodylate. calcium acetate