5C6C
PKG II's Amino Terminal Cyclic Nucleotide Binding Domain (CNB-A) in a complex with cAMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E+ SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2013-12-09 |
Detector | RIGAKU RAXIS HTC |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.710, 63.760, 99.540 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.233 - 2.050 |
R-factor | 0.1919 |
Rwork | 0.186 |
R-free | 0.24260 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.046 |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 39.233 |
High resolution limit [Å] | 2.050 |
Number of reflections | 18656 |
<I/σ(I)> | 10.9 |
Completeness [%] | 96.7 |
Redundancy | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 296 | 200 mM calcium chloride, 200 mM cadmium chloride, 200 mM cobalt (II) chloride, 20% polyethylene glycol 3350 |