5C6B

Crystal Structure of Prefusion-stabilized RSV F variant SC-TM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2014-11-11
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792368
Spacegroup name	P 41 3 2
Unit cell lengths	168.200, 168.200, 168.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.950 - 2.400
R-factor	0.1797
Rwork	0.178
R-free	0.21100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4mms
RMSD bond length	0.003
RMSD bond angle	0.700
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.950	2.490
High resolution limit [Å]	2.400	2.400
Rmerge		1.327
Number of reflections	32205
<I/σ(I)>	12.6	1.9
Completeness [%]	99.7	100
Redundancy	10.5	10.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	9.5	293	1.34M K/Na tartrate, 0.2M LiSO4, 0.1M CHES pH 9.5