5C5S

Crystal Structure of human Myosin 9b RhoGAP domain at 2.2 angstrom

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-24
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	77.084, 84.984, 134.139
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.570 - 2.200
R-factor	0.2225
Rwork	0.221
R-free	0.24590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ow3
RMSD bond length	0.003
RMSD bond angle	0.706
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.320
High resolution limit [Å]	2.200	2.200
Rmerge	0.113	0.615
Number of reflections	45424
<I/σ(I)>	10.5	3
Completeness [%]	99.9	100
Redundancy	6.9	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	298	0.2 M NH4Ac, 0.1 M Bis-Tris and 20% w/v PEG 3350