5C5J

Poymerase Nucleotide complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2012-11-05
Detector	MARRESEARCH
Wavelength(s)	0.91596
Spacegroup name	P 1 21 1
Unit cell lengths	86.450, 57.380, 110.640
Unit cell angles	90.00, 94.51, 90.00

Refinement procedure

Resolution	36.909 - 2.100
R-factor	0.225
Rwork	0.222
R-free	0.27440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ir9
RMSD bond length	0.009
RMSD bond angle	1.078
Data reduction software	SCALA
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.909	2.210
High resolution limit [Å]	2.100	2.100
Rmerge	0.069	0.606
Number of reflections	63454
<I/σ(I)>	11.2	2.4
Completeness [%]	99.8	100
Redundancy	4.4	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	298	MPD