5C56

Crystal structure of USP7/HAUSP in complex with ICP0

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2013-01-08
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97852
Spacegroup name	P 21 21 21
Unit cell lengths	78.879, 80.378, 151.149
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.460 - 2.685
R-factor	0.219
Rwork	0.215
R-free	0.26300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ylm
RMSD bond length	0.010
RMSD bond angle	1.478
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.800
High resolution limit [Å]	2.685	5.810	2.685
Rmerge	0.096	0.045	0.866
Rmeas	0.105	0.050	0.945
Rpim	0.042	0.021	0.374
Total number of observations	166411
Number of reflections	27389
<I/σ(I)>	10.6
Completeness [%]	99.8	98.4	100
Redundancy	6.1	5.6	6.2
CC(1/2)		0.996	0.782

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	20% PEG3350, 0.2M Sodium Bromide