5C52

Probing the Structural and Molecular Basis of Nucleotide Selectivity by Human Mitochondrial DNA Polymerase gamma

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2014-08-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 41 21 2
Unit cell lengths	217.277, 217.277, 165.865
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	51.213 - 3.637
R-factor	0.3112
Rwork	0.311
R-free	0.31370
RMSD bond length	0.004
RMSD bond angle	0.826
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	51.213
High resolution limit [Å]	3.560
Number of reflections	47896
<I/σ(I)>	2
Completeness [%]	97.0
Redundancy	9.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	300	PEG4000, KCl, HEPES