5C4Y
Crystal structure of putative TetR family transcription factor from Listeria monocytogenes
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-07-02 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97915 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 195.697, 52.169, 50.403 |
Unit cell angles | 90.00, 104.97, 90.00 |
Refinement procedure
Resolution | 33.838 - 1.770 |
R-factor | 0.1643 |
Rwork | 0.162 |
R-free | 0.20620 |
Structure solution method | SAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.578 |
Data reduction software | HKL-3000 |
Data scaling software | SCALEPACK |
Phasing software | HKL-3000 |
Refinement software | PHENIX ((dev_2056: ???)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.800 |
High resolution limit [Å] | 1.770 | 4.800 | 1.770 |
Rmerge | 0.054 | 0.022 | 0.422 |
Total number of observations | 297155 | ||
Number of reflections | 47069 | ||
<I/σ(I)> | 11.6 | ||
Completeness [%] | 98.6 | 97.7 | 87.5 |
Redundancy | 6.3 | 6.3 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289 | 12% PEG6000, 2.0M sodium chlorode |