5C4R
CobK precorrin-6A reductase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-06-28 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.13956 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 33.900, 75.300, 115.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 63.010 - 3.170 |
| R-factor | 0.18194 |
| Rwork | 0.179 |
| R-free | 0.23595 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.597 |
| Data scaling software | Aimless |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.010 | 3.340 |
| High resolution limit [Å] | 3.170 | 3.170 |
| Rmerge | 0.596 | |
| Number of reflections | 16610 | |
| <I/σ(I)> | 5.5 | 2.6 |
| Completeness [%] | 98.2 | 97 |
| Redundancy | 1.8 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 291 | 18% PEG3350, 0.2M NH4Cl |
