5C4M
RhoA GDP with novel switch II conformation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-04-10 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.976 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 31.796, 66.271, 83.679 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.300 - 1.300 |
R-factor | 0.17147 |
Rwork | 0.170 |
R-free | 0.19953 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.343 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.300 |
High resolution limit [Å] | 1.300 |
Number of reflections | 44107 |
<I/σ(I)> | 20.3 |
Completeness [%] | 99.5 |
Redundancy | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 24% (w/v) PEG 3350, 0.3 NaCl |