5C41
Crystal structure of human ribokinase in complex with AMPPCP in P21 spacegroup and with 4 protomers
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12C |
Synchrotron site | NSLS |
Beamline | X12C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-06-16 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 51.950, 89.190, 144.400 |
Unit cell angles | 90.00, 98.16, 90.00 |
Refinement procedure
Resolution | 44.550 - 1.950 |
R-factor | 0.17394 |
Rwork | 0.172 |
R-free | 0.20733 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2fv7 |
RMSD bond length | 0.020 |
RMSD bond angle | 2.011 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.550 | 2.000 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.096 | 0.727 |
Number of reflections | 94826 | |
<I/σ(I)> | 11.1 | 1.9 |
Completeness [%] | 99.8 | 98.8 |
Redundancy | 3.8 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 0.1M sodium HEPES, 10% PEG 8000 |