5C3X
Crystal structure of Staphylococcal nuclease variant Delta+PHS I72D/N100E at cryogenic temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OTHER |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2014-01-16 |
| Detector | APEX II CCD |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.126, 60.173, 38.047 |
| Unit cell angles | 90.00, 95.03, 90.00 |
Refinement procedure
| Resolution | 37.900 - 1.950 |
| R-factor | 0.1683 |
| Rwork | 0.166 |
| R-free | 0.20770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bdc |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.877 |
| Data reduction software | SAINT |
| Data scaling software | SAINT |
| Phasing software | PHASER (2.5.2) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.980 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.020 | 0.119 |
| Total number of observations | 247526 | |
| Number of reflections | 10271 | |
| <I/σ(I)> | 36.06 | 7.89 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 23.95 | 17.57 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 30% MPD, 25 mM potassium phosphate, calcium chloride, pdTp |
