5C3S
Crystal structure of the full-length Neurospora crassa T7H in complex with alpha-KG and 5-formyluracil (5fU)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-30 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.9199 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.801, 155.604, 75.954 |
| Unit cell angles | 90.00, 91.74, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.150 |
| R-factor | 0.1861 |
| Rwork | 0.184 |
| R-free | 0.22780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5c3o |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.058 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.230 |
| High resolution limit [Å] | 2.150 | 4.630 | 2.150 |
| Rmerge | 0.123 | 0.065 | 0.594 |
| Rmeas | 0.140 | 0.074 | 0.680 |
| Rpim | 0.068 | 0.035 | 0.330 |
| Total number of observations | 290491 | ||
| Number of reflections | 69438 | ||
| <I/σ(I)> | 8.4 | 3.7 | |
| Completeness [%] | 98.5 | 99.4 | 97.1 |
| Redundancy | 4.2 | 4.3 | 4.1 |
| CC(1/2) | 0.995 | 0.758 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 289 | 0.1 M BIS-TRIS, 25%(W/v) PEG 3350 |
