5C3Q

Crystal structure of the full-length Neurospora crassa T7H in complex with alpha-KG and thymine (T)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-05-18
Detector	RAYONIX MX225HE
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	56.900, 156.318, 76.028
Unit cell angles	90.00, 91.77, 90.00

Refinement procedure

Resolution	50.000 - 2.050
R-factor	0.1856
Rwork	0.184
R-free	0.21480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5c3o
RMSD bond length	0.005
RMSD bond angle	0.977
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.120
High resolution limit [Å]	2.050	4.420	2.050
Rmerge	0.085	0.047	0.468
Total number of observations	314882
Number of reflections	82717
<I/σ(I)>	10.1		3
Completeness [%]	99.9	99.1	100
Redundancy	3.8	3.7	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	289	0.1 M BIS-TRIS, 25%(W/v) PEG 3350