5C3P

Crystal structure of the full-length Neurospora crassa T7H in complex with alpha-KG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-11-24
Detector	RAYONIX MX225HE
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	57.185, 154.726, 75.434
Unit cell angles	90.00, 90.60, 90.00

Refinement procedure

Resolution	50.000 - 2.100
R-factor	0.1872
Rwork	0.185
R-free	0.22190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5c3o
RMSD bond length	0.006
RMSD bond angle	1.077
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.180
High resolution limit [Å]	2.100	4.520	2.100
Rmerge	0.079	0.058	0.406
Rmeas	0.095	0.070	0.485
Rpim	0.052	0.039	0.263
Total number of observations	249555
Number of reflections	75367
<I/σ(I)>	10.9		3.2
Completeness [%]	99.3	96.8	99.9
Redundancy	3.3	3.2	3.3
CC(1/2)		0.990	0.839

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	289	0.1 M BIS-TRIS, 25%(W/v) PEG 3350