5C3L
Structure of the metazoan Nup62.Nup58.Nup54 nucleoporin complex.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-08-24 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97864 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 167.570, 167.570, 142.720 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.878 - 2.900 |
| R-factor | 0.2658 |
| Rwork | 0.265 |
| R-free | 0.30070 |
| Structure solution method | SAD |
| Starting model (for MR) | 4hep |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.922 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 51.000 | 2.870 | |
| High resolution limit [Å] | 2.800 | 12.520 | 2.800 |
| Rmerge | 0.235 | 0.040 | 6.106 |
| Rmeas | 0.236 | 0.040 | 6.123 |
| Total number of observations | 5534630 | ||
| Number of reflections | 49303 | 404 | 2058 |
| <I/σ(I)> | 39.44 | 152.14 | 1.66 |
| Completeness [%] | 98.8 | 97.6 | 96.3 |
| Redundancy | 101 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.4 | 293 | PEG MME 2000, Trimethylamine N-oxide, Tris-HCl |
