5C3C
Structural characterization of a newly identified component of alpha-carboxysomes: The AAA+ domain Protein cso-CbbQ
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-08-28 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.00003 |
Spacegroup name | H 3 |
Unit cell lengths | 171.913, 171.913, 53.565 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 38.798 - 2.800 |
R-factor | 0.2313 |
Rwork | 0.226 |
R-free | 0.28300 |
Structure solution method | SAD |
RMSD bond length | 0.004 |
RMSD bond angle | 0.900 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1690) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.800 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.113 | 0.314 |
Number of reflections | 14510 | |
<I/σ(I)> | 13 | |
Completeness [%] | 99.9 | 100 |
Redundancy | 4.8 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | 5 mg/ml protein, 0.1 M HEPES, 8 % ethylene glycol, 6% PEG-8000 |