5C2K

Crystal structure of the fusion protein linked by RhoA and the GAP domain of MgcRacGAP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	110
Detector technology	CCD
Collection date	2012-10-05
Detector	MARRESEARCH
Wavelength(s)	1.00
Spacegroup name	P 1 21 1
Unit cell lengths	44.491, 77.041, 62.253
Unit cell angles	90.00, 108.53, 90.00

Refinement procedure

Resolution	32.260 - 1.420
R-factor	0.184
Rwork	0.184
R-free	0.20600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ovj 1tx4
RMSD bond length	0.004
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.470
High resolution limit [Å]	1.420	1.420
Number of reflections	73586
<I/σ(I)>	21.9	3.4
Completeness [%]	99.1	94.7
Redundancy	4.2	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	PEG3350, Bis-Tris