5C2D
K428A mutant gp2c of large terminase subunit from bacteriophage sf6 with calcium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-11-25 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 1.03361 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 133.078, 57.437, 46.568 |
Unit cell angles | 90.00, 98.99, 90.00 |
Refinement procedure
Resolution | 18.946 - 1.590 |
R-factor | 0.1613 |
Rwork | 0.160 |
R-free | 0.19150 |
Data scaling software | XSCALE |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.600 | 1.620 |
High resolution limit [Å] | 1.590 | 1.590 |
Rmerge | 0.052 | 0.814 |
Number of reflections | 46066 | |
<I/σ(I)> | 16.1 | 1.2 |
Completeness [%] | 98.6 | 90.7 |
Redundancy | 3 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 100mM HEPES, pH7.5, 50mM NaCl, 8% PEG8000 |