5C19

p97 variant 2 in the apo state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-06-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9184
Spacegroup name	P 21 21 21
Unit cell lengths	137.665, 165.780, 243.992
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.129 - 4.200
R-factor	0.2402
Rwork	0.237
R-free	0.30230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3cf3
RMSD bond length	0.002
RMSD bond angle	0.597
Data scaling software	Aimless (0.3.8)
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.130	49.130	4.370
High resolution limit [Å]	4.200	15.140	4.200
Rmerge	0.259	0.044	1.197
Rpim	0.107	0.021	0.484
Total number of observations	277534	5075	32400
Number of reflections	41484
<I/σ(I)>	7.3	30.6	1.8
Completeness [%]	99.9	96.3	100
Redundancy	6.7	5.3	7
CC(1/2)	0.992	0.997	0.680

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	12% PEG 4000, 0.15 M ammonium sulfate, 0.1 M MES pH 6.5