5C13

Crystal Structure of TAF3 PHD finger bound to histone H3C4me3 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-16
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9791
Spacegroup name	P 1 21 1
Unit cell lengths	30.212, 50.105, 85.949
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.620 - 2.101
R-factor	0.2248
Rwork	0.222
R-free	0.27960
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.557
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.140
High resolution limit [Å]	2.100	5.700	2.100
Rmerge	0.123	0.054	0.669
Total number of observations	54999
Number of reflections	15064
<I/σ(I)>	7.1
Completeness [%]	99.6	98.4	100
Redundancy	3.7	3.5	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	0.03M magnesium chloride, 0.03M calcium chloride, 0.1M MES, 0.1M imidazole, PH6.5, 15% PEGMME 550, 15% PEG 20K