5C13
Crystal Structure of TAF3 PHD finger bound to histone H3C4me3 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 30.212, 50.105, 85.949 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.620 - 2.101 |
| R-factor | 0.2248 |
| Rwork | 0.222 |
| R-free | 0.27960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.557 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 5.700 | 2.100 |
| Rmerge | 0.123 | 0.054 | 0.669 |
| Total number of observations | 54999 | ||
| Number of reflections | 15064 | ||
| <I/σ(I)> | 7.1 | ||
| Completeness [%] | 99.6 | 98.4 | 100 |
| Redundancy | 3.7 | 3.5 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 0.03M magnesium chloride, 0.03M calcium chloride, 0.1M MES, 0.1M imidazole, PH6.5, 15% PEGMME 550, 15% PEG 20K |
