5C10

Nuclease domain of the large terminase subunit gp2 of bacterial virus Sf6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2013-08-18
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.03316
Spacegroup name	C 1 2 1
Unit cell lengths	133.683, 57.734, 46.654
Unit cell angles	90.00, 98.86, 90.00

Refinement procedure

Resolution	19.044 - 1.550
R-factor	0.1455
Rwork	0.144
R-free	0.16470
RMSD bond length	0.009
RMSD bond angle	1.179
Data scaling software	Aimless (0.1.29)
Phasing software	PHENIX ((phenix.refine: 1.8.1_1168))
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.690	29.690	1.570
High resolution limit [Å]	1.550	8.470	1.550
Rmerge	0.040	0.033	0.608
Rpim	0.025	0.024	0.370
Total number of observations	184520	996	8045
Number of reflections	50109
<I/σ(I)>	17.7	48.7	1.9
Completeness [%]	97.7	91.9	90.8
Redundancy	3.7	3.2	3.5
CC(1/2)	0.999	0.994	0.841

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	100mM HEPES, pH7.5, 50mM NaCl, 8% PEG8000