5C0X

Structure of a 12-subunit nuclear exosome complex bound to structured RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-03-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.2398
Spacegroup name	P 21 21 21
Unit cell lengths	106.150, 177.390, 299.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	57.887 - 3.812
R-factor	0.2949
Rwork	0.295
R-free	0.29960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ifd 2hbj
RMSD bond length	0.004
RMSD bond angle	0.704
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	57.887		4.200
High resolution limit [Å]	3.810	20.000	3.810
Rmerge	0.163	0.091	0.843
Rmeas	0.175	0.098	1.081
Total number of observations	256794
Number of reflections	45806	441	5499
<I/σ(I)>	4.83	21.7	0.69
Completeness [%]	80.8	94.6	38
Redundancy	5.6		1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	283	7%(w/v) PEG 8000, 0.1M MES pH 6.5, 0.15M NaCl, 5%(v/v) Glycerol, 283K or 292K