5C0P
The crystal structure of endo-arabinase from Bacteroides thetaiotaomicron VPI-5482
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-25 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 43 |
| Unit cell lengths | 83.804, 83.804, 43.366 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.133 - 1.532 |
| R-factor | 0.1498 |
| Rwork | 0.149 |
| R-free | 0.16380 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.161 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MLPHARE |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.560 |
| High resolution limit [Å] | 1.530 | 1.530 |
| Rmerge | 0.078 | 0.486 |
| Number of reflections | 45139 | |
| <I/σ(I)> | 18.7 | 1.3 |
| Completeness [%] | 99.2 | 88.8 |
| Redundancy | 3.7 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 286 | 0.16M MgCl2, 0.08M Tris:HCl, 24% (w/v) PEG4000, 15(v/v)Glycerol |
