5C0O
m1A58 tRNA methyltransferase mutant - Y78A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.872 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 76.540, 79.860, 184.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 52.923 - 2.620 |
| Rwork | 0.235 |
| R-free | 0.28240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2pwy |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.21) |
| Phasing software | PHASER (2.5.6) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 52.920 | 52.923 | 2.630 |
| High resolution limit [Å] | 2.620 | 7.880 | 2.620 |
| Rmerge | 0.042 | 0.539 | |
| Rmeas | 0.104 | ||
| Rpim | 0.055 | 0.027 | 0.346 |
| Total number of observations | 139384 | 4627 | 18012 |
| Number of reflections | 34672 | ||
| <I/σ(I)> | 10.78 | 21.7 | 2 |
| Completeness [%] | 99.9 | 98.6 | 96 |
| Redundancy | 3.5 | 3.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 292 | 2.1 M ammonium sulfate, 8% isopropanol |
